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Watch the Full Video at https://www.jove.com/v/62046/characte.... Characterization of a Novel Human Organotypic Retinal Culture Technique - a 2 minute Preview of the Experimental Protocol Charisse Y. J. Kuo, Henry H. Louie, Ilva D. Rupenthal, Odunayo O. Mugisho University of Auckland, Buchanan Ocular Therapeutics Unit, Department of Ophthalmology and the New Zealand National Eye Centre; This study aims to develop a novel human organotypic retinal culture (HORC) model that prevents compromising retinal integrity during explant handling. This is achieved by culturing the retina with the overlying vitreous and the underlying retinal pigment epithelium-choroid (RPE-choroid) and sclera. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments