Electroporation Based Gene Transfer for Analysis of Granule cells | Protocol Preview скачать в хорошем качестве

Electroporation Based Gene Transfer for Analysis of Granule cells | Protocol Preview

Watch the Full Video at https://www.jove.com/v/57311/crispr-m.... CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer - a 2 minute Preview of the Experimental Protocol Weijun Feng, Lena Herbst, Peter Lichter, Stefan M. Pfister, Hai-Kun Liu, Daisuke Kawauchi German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance, Division of Molecular Neurogenetics; German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK), Division of Molecular Genetics; Hopp-Children's Cancer Center at the NCT Heidelberg (KiTZ),; German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK), Division of Pediatric Neurooncology; Heidelberg University Hospital, Department of Pediatric Hematology and Oncology; Conventional loss-of-function studies of genes using knockout animals have often been costly and time-consuming. Electroporation-based CRISPR-mediated somatic mutagenesis is a powerful tool to understand gene functions in vivo. Here, we report a method to analyze knockout phenotypes in proliferating cells of the cerebellum. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments