Bielschowsky’s stain silver staining technique to visualize nerve fibers, neurofibrillary tangles скачать в хорошем качестве

Bielschowsky’s stain silver staining technique to visualize nerve fibers, neurofibrillary tangles

Bielschowsky's stain is a silver staining technique used in histology to visualize nerve fibers, neurofibrillary tangles, and senile plaques, particularly in the study of neurological disorders like Alzheimer's disease. Here's an overview: Principle The stain involves silver impregnation, where nerve fibers are sensitized with a silver solution. Ammoniacal silver is then applied, and the silver is reduced to metallic silver, making the structures visible under a microscope. Applications Neurological Research: Used to study neurodegenerative diseases, including Alzheimer's disease, by highlighting neurofibrillary tangles and plaques. Histological Analysis: Helps visualize axons and other nerve structures in tissue sections. Procedure Tissue sections are fixed in formalin and prepared on slides. Silver nitrate solution is applied to sensitize the tissue. Ammoniacal silver solution is used for impregnation. A developer solution reduces the silver to metallic silver. Unreacted silver is removed using sodium thiosulfate. Results Neurofibrillary Tangles: Appear black. Senile Plaques: Appear brown. Background: Typically gold or light-colored. #stain