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This animation provides an explanation for how regression analysis can be used to examine the relationship between two or more variables. This video is part of a series of CLOSER animations introducing different statistical methods and methodological approaches to analyse longitudinal data. The four animations in this series focus on the following topics: Introduction to analysing longitudinal data Regression analysis Multi-level and growth curve analysis Survival analysis CLOSER Learning Hub These animations are featured on the CLOSER Learning Hub (https://learning.closer.ac.uk), our online educational resource which aims to help those new to longitudinal studies better understand the value of these studies and how to use the data. About CLOSER CLOSER (https://closer.ac.uk) is a consortium of world-leading longitudinal studies following participants born throughout the 20th and 21st centuries. Our work maximises the use, value and impact of these, and other, longitudinal studies to help improve our understanding of key social and biomedical challenges.