What is Tissue Culture Micropropagation? скачать в хорошем качестве

What is Tissue Culture Micropropagation?

What is micropropagation? What is tissue culture? I answer these questions (and more) in a video that I'm hoping is at least watchable. Enjoy! Feel free to ask me any questions you have about the process. ... TRANSCRIPT: Hey guys, Bret here. Today, I’m going to explain what micropropagation is and show you how it works. Alright, let’s start. [title card: What is Micropropagation?] If you’ve ever heard of in-vitro propagation, just know that it’s the same thing. That being said, what is Micropropagation? According to dictionary.com, the definition of micropropagation—or, you know, in-vitro propagation, or whatever you want to call it—what that refers to is the process of taking bits of tissue from a plant and using them to create more plants. Micropropagation can use parts of a plant as small as a single cell, although we definitely use larger tissue samples in our lab. Here, I’ll show you how we do Tissue Culture. There are other types of Micropropagation, but we can talk about that in another video. [title card: Excise material] V.O. over montage of me washing the rosemary in soapy water, bleach, and D.I. water. I’ll begin by gathering plant material from some of the plants we have sitting around. Right now, I’m going to practice on this rosemary plant.  Of course, everything needs to be as clean as possible. I’ve already sanitized my work surface, but this rosemary needs to be clean, too. I’m rinsing it all with soapy water, bleach, and sterile distilled water. [title card: Divide material] V.O. over montage of test tubes and then me dividing the material Brian, who runs the lab, already made a bunch of test tubes and filled them with growing medium.  Right now, the recipe we’re using is based on the famous Murashige and Skoog medium. It’s similar to a Japanese dessert in that it’s mostly agar gel and sugar, but we also add some chemicals are hormones that are designed to encourage the plants to put out shoots and leaves. Our test tubes are ready. Now, I just have to take the cleaned plant material and divide it into smaller pieces. We want to make sure that each section we cut has at least one node, which is what we call a joint in the stem. Nodes usually, but not always, have leaves growing out of them.  Because rosemary plants have so many nodes, they’re a really good plant to practice on. To prepare the plant material, we’ll start by cutting off all the leaves. We don’t need them, and they’re a source of contamination. After that, we want to cut off anything that looks visibly bleached. That tissue is already dead, and it’s just going to rot in the test tube. Now we just have the good stuff left. We need at least one node per section, but I’m going to  cut the pieces a little bit larger than I technically have to. Once they’re divided, I have to pick them up and put them in the test tube, which is really difficult to do if they’re too small. [title card: Place into culture] V.O. over footage of me placing the material into test tubes  Here are the test tubes! Thanks, Brian. After he filled the tubes, we waited a while to use them. You want to make sure to let them sit for at least three days to see if any fungi start growing in there. If nothing happens, the material is most likely sterile and should be fine to use for tissue culturing. Now, I’m going to carefully pick up each section and place it into the test tube. It’s really important to make sure that each section is right-side-up. Remember, what we want the plantlets to do is grow shoots and leaves. If we place them upside-down, they’ll actually start growing roots, which isn’t the end of the world but isn’t super useful, either. [title card: Let it  grow] V.O. over footage of the test tubes We’ll give each plantlet time to grow. Last month, I placed some tomatoes and other plants into tissue culture, and some of them have gotten pretty big now. [title card: Subdivide] V.O. over me subdividing Once the plantlets start to look a little too big for their test tubes, we have a few options. We can actually take them back into our sterile workspace and divide them again, or we can go ahead and place them into a different medium that will help them grow roots. In our lab, we always subdivide. It’s a great way to create more clones without using up any additional plant material.  Alright, let’s get going. Here we have some tomatoes. Because they came out of a clean tube, I don’t need to wash them in bleach, However, I am going to rinse them with sterile distilled water.  Now we’re going to do the same thing we did before and cut off those leaves. We’ll divide this into smaller sections and place each one back into tissue culture. [back to me] Technically, you can keep doing subdivision indefinitely, although the material can start to lose vigor over a few generations.  Anyway, that’s tissue culturing. Hope you enjoyed. Let me know in the comments if you have any questions or if there’s anything else you want to see!