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Quantitative estimation of blood glucose by GOD POD method

In this video, we perform a quantitative estimation of blood glucose levels using the enzymatic GOD-POD (Glucose Oxidase-Peroxidase) method. We cover the underlying biochemical principles, the reagents involved (GOD, POD, 4-aminoantipyrine, and phenol), the role of the standard glucose solution, and how to calculate the results using a colorimeter at 540 nm. This is an essential practical for MBBS and MD Biochemistry students. Topics covered: Principle of GOD-POD method (Trinder's Reaction) Role of individual reagents Preparation of the working reagent Procedure and incubation times Calculation using the standard formula Clinical significance and normal reference ranges 00:00 Introduction 00:14 Method 02:07 Materials needed 02:40 Sample needed 04:45 Capillary blood vs Venous blood 07:13 Reagents needed 07:48 Principle 09:03 Procedure 10:22 Calculation 10:42 Result 10:54 Interpretation 12:40 Hyperglycemia 15:45 Hypoglycemia 17:10 Other methods 17:35 Glucometer 21:52 Glucometer vs Lab method 24:03 Possible viva questions #MBBS #BiochemistryPractical #BloodGlucoseEstimation