How Reverse Transcriptase Affects Ct in RT-qPCR Gene Expression скачать в хорошем качестве

How Reverse Transcriptase Affects Ct in RT-qPCR Gene Expression

Learn how reverse transcriptase impacts Ct values in RT-PCR and RT-qPCR experiments: https://www.thermofisher.com/us/en/ho... This video explains what Ct is, how it reflects the starting RNA concentration, and how different reverse transcriptases can shift Ct results. With next-generation reverse transcriptases such as SuperScript IV RT, you can achieve more reliable cDNA synthesis and use less starting RNA, which is critical for low-input samples. Learn how to: 1. Define Ct (threshold cycle) in real-time PCR and relate it to target concentration. 2. Interpret Ct differences using 2^ΔCt to compare starting RNA levels between samples. 3. Recognize how the reverse transcription step influences Ct values in RT-qPCR. 4. Compare traditional and next-generation reverse transcriptases based on Ct performance. 5. Reduce input RNA requirements while maintaining sensitivity, especially for single-cell and FFPE samples. Chapters: 000:00 What Is Ct in Real-Time PCR? 00:25 Ct and Starting Target Concentration 00:55 Interpreting Ct Differences with 2^ΔCt 01:25 How Reverse Transcriptase Affects Ct 01:55 Benefits of Next-Generation Reverse Transcriptase 02:20 Using SuperScript IV RT for Reliable cDNA Synthesis #ThermoFisherScientific #qPCR #RTPCR #CtValues #ReverseTranscriptase #SuperScript4 #GeneExpression #MolecularBiology #cDNA #LabTraining