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Cell counting is a tedious process. You may have to count number of cells in many images. Manual cell counting is very laborious and time taking process. In this video we will see how we can use imageJ software in order to count the number of cells in an image. The process is pretty simple. The steps included are: 1. Conversion of image to 8-bit image 2. Adjusting the threshold 3. Making image binary 4. Converting to mask 5. Fill holes 6. Watershed 7. Analyze particles Main points that are discussed in this video are: 1. How to perform histometry by using ImageJ 2. How to do histometry by using Fiji 3. Understanding the concept of cell number counting 4. Analyze circularity in ImageJ/ Fiji 5. Area measurement in ImageJ/ Fiji 6. ImageJ tutorials 7. Automated image processing 8. ImageJ software 9. Image analysis with ImageJ 10. Image analysis with Fiji 11. Spatial measurement with ImageJ 12. Spatial measurement with Fiji _________________________________________________________________ ImageJ Calibration | Fiji Calibration | How to calibrate ImageJ Fiji (English) • ImageJ Calibration | Fiji Calibration | Ho... How to calibrate ImageJ (Fiji) | Scale bar in ImageJ (Fiji) (Urdu/हिंदी) • ImageJ Calibration | Fiji Calibration | Ho... How to add Scale Bar in ImageJ • How to add Scale Bar in ImageJ | ImageJ Tu... Scratch assay | Wound Healing Assay | Cell Migration Assay | ImageJ Software | Image Processing • Scratch assay | Wound Healing Assay | Cell... Quantify bands with ImageJ | Western blot analysis on SDS-PAGE |Quantify band intensity with ImageJ • Quantify bands with ImageJ | Western blot ... Updated tool: Wound Healing Assay | Scratch Assay | Cell Migration Assay • Updated tool: Wound Healing Assay | Scratc... Measurement of villus height and crypt depth with ImageJ | Morphometry of intestine • Measurement of villus height and crypt dep... This is a preliminary guide undergraduate and postgraduate students and researchers/ scientists. ImageJ is free software. It can be downloaded from: https://imagej.nih.gov/ij/download.html _________________________________________________________________ https://www.facebook.com/profile.php?... _________________________________________________________________ #imageJ #Fiji #cellcounting #cellnumber #areameasurement #Binary #Watershed #Fillholes #Threshold #immunofluorescence #immunohistochemistry #immunocytochemistry # positivecells #Quantification #automatedcellcounting #colonycounting #intensity #countobjects #countparticles #tutorial #lecture #quantification #nanoparticles #imageJcellcounter #pixel #pixels^2 #size #Circularity #Segmentation #CellSegmentation #CountingNuclei #Nuclei #BacterialColonies #image #adjust #8-bit #analyzeparticlesfunction #scanningelectronmicroscope #SEM #objects _________________________________________________________________