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In the previous lecture, we explored how computation meets biology across scales, from molecules to patients. In this lecture, Biological Data Story: From Question to File, we take a practical look at how biological data is created before it ever reaches a machine learning model. We walk through how a biological question becomes an experiment, how experiments generate raw signals, and how those signals are transformed into the digital files and formats that AI and machine learning engineers ultimately analyze. Understanding this data lineage is essential for debugging models, interpreting results, and recognizing hidden limitations in training data. Key Topics The Lifecycle of Biological Data Question → experiment → raw signal → file → model Where Scientists and Data Engineers Intersect How biological experiments translate into computational workflows Common Biological Data Formats Examples across molecular, cellular, and clinical scales How Datasets Are Shaped The roles of preprocessing, filtering, normalization, and aggregation Hidden Risks in Biological Data Batch effects, label construction, missing metadata, and experimental bias Series Context This course is designed to give AI and machine learning engineers the biological intuition needed to model modern therapeutics by understanding not just the file, but also the experiments and decisions that produced it.