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Watch the Full Video at https://www.jove.com/v/50011/processi.... Processing of Human Reduction Mammoplasty and Mastectomy Tissues for Cell Culture - a 2 minute Preview of the Experimental Protocol Mark A. LaBarge, James C. Garbe, Martha R. Stampfer Lawrence Berkeley National Laboratory, Life Science Division; A method to process human mammary surgical discard material is described. Processed tissue, in the form of organoids, can be stored frozen indefinitely or placed in culture for long-term growth. This method enables experimental examination of normal human epithelial cell biology, and the effects of exogenous perturbations. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments