Scientists try to create first water buffalo clone скачать в хорошем качестве

Scientists try to create first water buffalo clone

(5 May 2006) Nueva Ecija - 7 March 2006 3. Wide shot of water buffalo (carabao) eating 4. Water buffalos eating 5. Wide of ''high milk yielding'' water buffalo 6. Close-up of ''high milk yielding'' water buffalo 7. Wide of micro-manipulation lab 8. Close-up of video monitor showing somatic cells from ''high milk yielding'' water buffalo 9. Research specialist from the Philippine Carabao Centre Dr. Edwin Atabay pointing to somatic cells on video monitor 10. Petri dish containing somatic cells 11. SOUNDBITE: (English) Doctor Edwin Atabay, Senior Science Research Specialist, Philippine Carabao Centre: "We remove the nucleus or simply called enucleation of the recipient oocytes (a cell from which an egg or ovum develops by cell division), then replacing the nucleus from the donor cells. Then after injecting the donor cells from (into) the oocytes, then we use some machine to electrofuse, we simply call that electrofusion. After fusion, those couplets, we call these couplets, that fuse completely - we activate that by incubating it for six hours under certain chemicals, and after that we culture that in the carbon dioxide incubator for seven to eight days. Then after that, the embryos, the constructed embryos that contain the DNA that we want to produce can now be transferred to the recipient animals." 12. Atabay using microscope to view cultured embryos 13. Close-up of video monitor showing cultured embryos 14. Wide of surrogate dams (females) which are carrying the cultured embryos 15. Close-up of surrogate dams 16. SOUNDBITE: (English) Doctor Edwin Atabay, Senior Science Research Specialist, Philippine Carabao Centre: "There were some animals that showed...they skipped two cycles of oestrous (reproductive cycle), so we presumed then that the animals were pregnant. But after confirmation, the animals are empty, so we are just trying to see if there was an early embryonic death or some problems." Manila - 8 March 2006 17. Executive Director of the Philippine Carabao Centre Dr Libertado Cruz talking to staff 18. SOUNDBITE: (English) Doctor Libertado Cruz, Executive Director of Philippine Carabao Centre: "Well, I''m very confident that with the way we are progressing in our system, we will have the cloned buffalo or buffalos by next year." Nueva Ecija - 7 March 2006 19. Wide of farmer milking water buffalo 20. Water buffalo being milked 21. Close-up of milk 22. Farmers milking water buffalo Manila - 15 March 2006 23. SOUNDBITE: (English) Danilo Fausto, Talavera Dairy Cooperative: "I hope this will not be looked upon in a negative way, and looked upon as assistance to those who are in need. That''s why we welcome the discovery, because we know that the production of the cloned animal will be of great help to us and to our members." Nueva, Ecija - 7 March 2006 24. Wide shot of barn where water buffalos are eating hay 25. Water buffalos eating hay SUGGESTED LEAD IN: There are an estimated three million farmers in the Philippines and each owns a water buffalo, used to pull ploughs and till the ground. Now an agricultural institute in the Philippines is trying to create the world''s first clone of a water buffalo, or carabao. The cloning of animals has become easier and less expensive since the creation of Dolly the sheep in Scotland less than a decade ago. But scientists involved in this latest cloning project are still facing some challenges. STORYLINE: Scientists at the Philippine Carabao Centre in Nueva Ecija are hoping to produce the first water buffalo clone through somatic cell nuclear transfer technology. SOUNDBITE: (English) Find out more about AP Archive: http://www.aparchive.com/HowWeWork Twitter:   / ap_archive   Facebook:   / aparchives   ​​ Instagram:   / apnews   You can license this story through AP Archive: http://www.aparchive.com/metadata/you...