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Watch the Full Video at https://www.jove.com/v/59419/an-enzym.... An Enzyme-free Method for Isolation and Expansion of Human Adipose-derived Mesenchymal Stem Cells - a 2 minute Preview of the Experimental Protocol Lauren S. Sherman, Alexandra Condé-Green, Yahaira Naaldijk, Edward S. Lee, Pranela Rameshwar Rutgers Biomedical and Health Sciences, Department of Medicine, Division of Hematology/Oncology, New Jersey Medical School; Rutgers Biomedical and Health Sciences, Rutgers School of Graduate Studies at New Jersey Medical School; Rutgers Biomedical and Health Sciences, Department of Surgery, Division of Plastic Surgery, New Jersey Medical School; This protocol provides an enzyme-free method for isolating mesenchymal stem cells from abdominoplasty and lipoaspirate samples using an explant method. The absence of harsh enzymes or centrifugation steps provides for clinically relevant stem cells that can be used for studies in vitro or transferred back to the clinic. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments