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Watch the Full Video at https://www.jove.com/v/50047/quantita.... Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy - a 2 minute Preview of the Experimental Protocol Chun A. Changou, Deanna L. Wolfson, Balpreet Singh Ahluwalia, Richard J. Bold, Hsing-Jien Kung, Frank Y.S. Chuang University of California, Davis , Department of Biochemistry and Molecular Medicine; University of California, Davis , NSF Center for Biophotonics Science & Technology; University of Tromsø,; University of California, Davis , Department of Surgery (Division of Surgical Oncology); University of California, Davis , UC Davis Comprehensive Cancer Center; University of California, Davis , Department of Biological Chemistry; Autophagy is a ubiquitous process that enables cells to degrade and recycle proteins and organelles. We apply advanced fluorescence microscopy to visualize and quantify the small, but essential, physical changes associated with the induction of autophagy, including the formation and distribution of autophagosomes and lysosomes, and their fusion into autolysosomes. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments