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Polymerase Chain Reaction (PCR) Protocol

Do you have a short region of a DNA molecule that needs to be copied? Alyssa, a QC Scientist at Addgene, walks you through the PCR process. For the full protocol text, visit https://www.addgene.org/protocols/pcr/ A standard Polymerase Chain Reaction (PCR) is an in vitro method that allows a single, short region of a DNA molecule (single gene perhaps) to be copied multiple times by Taq Polymerase. From a single copy of DNA (the template), a researcher can create thousands of identical copies using a simple set of reagents and a basic heating and cooling (denaturing and annealing) cycle. The process became automated with the discovery of a heat resistant DNA polymerase from the thermophilic bacterium, Thermus aquaticus (Taq). Taq polymerase can withstand many heating and cooling cycles, which would denature DNA polymerases from other species. ___________________________________________________________________________ 0:00 Intro & Overview 1:35 Materials 2:29 Getting Started in the Lab 3:20 PCR Process 5:01 Agarose Gel Prep & Downtime 5:15 Running Your Gel 5:30 Troubleshooting ___________________________________________________________________________ Credits Featuring: Alyssa Cecchetelli Directed, Animated, Edited, & Written by: Quintin Marcelino Music by: Lauren Duski, Vibe Mountain, and RKVC, courtesy of Youtube Audio Library