Tumor-stromal Interaction by 3D Co Culture Model | Protocol Preview скачать в хорошем качестве

Tumor-stromal Interaction by 3D Co Culture Model | Protocol Preview

Watch the Full Video at https://www.jove.com/v/52469/three-di.... Three-dimensional Co-culture Model for Tumor-stromal Interaction - a 2 minute Preview of the Experimental Protocol Masafumi Horie, Akira Saito, Yoko Yamaguchi, Mitsuhiro Ohshima, Takahide Nagase The University of Tokyo, Department of Respiratory Medicine, Graduate School of Medicine; The University of Tokyo, Department of Clinical Laboratory, Graduate School of Medicine; The University of Tokyo, Division for Health Service Promotion; Nihon University School of Dentistry, Department of Biochemistry; Ohu University School of Pharmaceutical Sciences, Department of Biochemistry; Here we present a protocol to co-culture in three-dimensions, which is useful for investigating multicellular interactions and extracellular matrix-dependent modulation of cancer cell behavior. In this experimental model, cancer cells are cultured on collagen gels embedded with human cancer-associated fibroblasts. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments