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Watch the Full Video at https://www.jove.com/v/62274/cryo-str.... Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells - a 2 minute Preview of the Experimental Protocol Nina Vyas, Nina Perry, Chidinma A. Okolo, Ilias Kounatidis, Thomas M. Fish, Kamal L. Nahas, Archana Jadhav, Mohamed A. Koronfel, Johannes Groen, Eva Pereiro, Ian M. Dobbie, Maria Harkiolaki Beamline B24, Diamond Light Source, Harwell Science and Innovation Campus; University of Cambridge, Division of Virology, Department of Pathology; Beamline 09 - MISTRAL, ALBA Synchrotron; University of Oxford, Micron Advanced Imaging Consortium, Department of Biochemistry; This protocol demonstrates how to image biological cryo-preserved samples using cryo-structured illumination microscopy. We demonstrate the methodology by imaging the cytoskeleton of U2OS cells. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments