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Watch the Full Video at https://www.jove.com/v/59664/fast-eff.... Fast and Efficient Expression of Multiple Proteins in Avian Embryos Using mRNA Electroporation - a 2 minute Preview of the Experimental Protocol Martin Tran, Mohit Dave, Rusty Lansford University of Southern California, Los Angeles, Department of Biological Sciences; Children's Hospital Los Angeles, Department of Radiology and Developmental Neuroscience Program, Saban Research Institute; University of Southern California, Los Angeles, Keck School of Medicine, Department of Radiology; We report messenger RNA (mRNA) electroporation as a method that permits fast and efficient expression of multiple proteins in the quail embryo model system. This method can be used to fluorescently label cells and record their in vivo movements by time-lapse microscopy shortly after electroporation. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments