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Watch the Full Video at https://www.jove.com/v/53465/preparat.... Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering - a 2 minute Preview of the Experimental Protocol Morito Sakuma, Yoshikazu Kumashiro, Masamichi Nakayama, Nobuyuki Tanaka, Yuji Haraguchi, Kazuo Umemura, Tatsuya Shimizu, Masayuki Yamato, Teruo Okano Tokyo University of Science, Department of Physics; Tokyo Women's Medical University, Institute of Advanced Biomedical Engineering and Science; Nanoscaled sea-island surfaces composed of thermoresponsive block copolymers were fabricated by the Langmuir-Schaefer method for controlling spontaneous cell adhesion and detachment. Both the preparation of the surface and the adhesion and detachment of cells on the surface were visualized. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments