How to Annotate T-Cell Receptor (TCR) Specificity from CellRanger Data скачать в хорошем качестве

How to Annotate T-Cell Receptor (TCR) Specificity from CellRanger Data

Learn how to bridge the gap between 10x Genomics Cell Ranger outputs and functional TCR insights. In this tutorial, Sander from ImmuneWatch demonstrates how to use ImmuneWatch DETECT to annotate the specificity of T-cell receptors (TCRs) directly from your single-cell sequencing experiments. We walk through the entire workflow — from locating your air_rearrangements.tsv file in the CellRanger VDJ folder to generating a fully annotated list of predicted epitopes, antigens, and MHC molecules. 🕒 Key Chapters: 0:00 Intro: Moving from Cell Ranger to TCR Specificity 0:15 Reviewing Cell Ranger Web Summary & VDJ Data 0:50 Locating the AIRR Rearrangements Output File 0:15 How to Install ImmuneWatch DETECT via Docker 0:50 Requesting Your Free License Key 0:10 Running the Command: Inputting Paired Alpha/Beta Chains 0:20 Analyzing the Results: Epitope Scores, Antigens, and MHC 0:45 Sorting Results and Downstream Analysis (Excel, R, Python) 🚀 Get Started: Download ImmuneWatch DETECT: https://immunewatch.gitlab.io/detect-... Request Your License Key: https://www.immunewatch.com/detect Or book our TCR repertoire analysis service so that experts can analyse the data for you: https://www.immunewatch.com/immunoinf... 🔬 Why use ImmuneWatch DETECT? ImmuneWatch DETECT automatically recognises paired alpha and beta chains linked by Cell ID to provide a single, high-confidence prediction for each TCR. Whether you are researching SARS-CoV-2, influenza, or tumor-specific TCRs, this tool helps you identify what your cells are actually targeting. Subscribe for more T-cell analysis tutorials! #TCR #SingleCellSequencing #CellRanger #ImmuneWatch #Bioinformatics #Immunology #TCellReceptor #10xGenomics