Nanotalk - Time resolved TEM to study the assembly of macromolecular & hybrid structures in solution скачать в хорошем качестве

Nanotalk - Time resolved TEM to study the assembly of macromolecular & hybrid structures in solution

In this nanotalk, Prof. Nico Sommerdijk from the Radboud University Medical Center, Nijmegen, The Netherlands, presented various research examples that illustrate how time resolved TEM can be used to visualize the evolution of morphology and structure in solution, from the (sub)nanometer level up. 00:52 Introduction to the presenter 02:15 presentation 02:46 Bone biomineralization 08:33 Liquid Phase Electron Microscopy (LPEM) 13:04 LPEM for soft matter structures 13:54 DENSsolutions Ocean system 21:31 LPEM for biological materials 23:18 Graphene liquid cells Q&A: 27:05 LPEM limitation for small biological molecules 29:52 Electron dose for vesicle formation study 32:21 Cryo-TEM vs LPEM electron beam damage 35:04 Introducing liquids by diffusion 37:42 Camera usage 39:26 Graphene sheets mounting Abstract: Inspired by the high complexity and functionality achieved in biological systems many researchers use macromolecular self-assembly and bioinspired crystallization to generate hierarchically structured materials. To control the formation of these structures it is of importance to understand the interactions between the different building blocks at the nanoscopic level. Various examples will be presented that illustrate how we use time resolved transmission electron microscopy (TEM) to visualize the evolution of morphology and structure in solution from the (sub)namometer level up. Using low dose imaging techniques originating from the life science TEM community we investigate soft matter structure formation processes using self-assembly and crystallization. Here we compare cryogenic TEM (cryoTEM), which provides high spatial resolution in 2D and 3D but limited temporal resolution, with liquid phase EM (LP-EM) that provides dynamic 2D information, but often limited spatial resolution. Beyond the investigation of synthetic soft matter there are now exiting examples of the use of LP-EM for biological materials. We will discuss recent technological advances and strategies that we think will open the way to the dynamic nanoscale imaging of this broad and important class of materials.