Lipocube SVM process. скачать в хорошем качестве

Lipocube SVM process.

Adipose-derived Stromal Vascular Matrix (SVM): a new paradigm in regenerative medicine. The conventional method of harvesting Stromal Vascular Fraction (SVF) from adipose tissue is an enzymatic digestion of the extra-cellular matrix (ECM) from the lipoaspirate. This process necessarily affects the viability and potency of the cells and eliminates the majority of pericytes embedded in the fibrous matrix and structural/functional support of ECM. The preparation of SVM using the LipocubeTM mechanical digestion technology results in a source of autologous and minimally manipulated adipose-derived stem cells for use in cosmetic and regenerative medicine procedures. Cell counts, viability assessments, CD antigen expression and gene expression analysis showed that the SVM is an optimal product for therapy. Conclusions: The SVM has a higher regenerative cell potency and provides greater ECM support compared to common enzymatic digestion methods. In clinical applications, SVM seems to be suitable for suboptimal recipient conditions and skin regeneration purposes. Obtaining and resuspending the mechanically obtained SVF and the matrix-rich lipoaspirate buffy coat together as SVM, delivers comparable cell yields to enzymatic digestion and has a higher re- generative cell potential. In clinical applications, SVM seems to be suitable for suboptimal recipient conditions and skin regeneration purposes. The SVM has a higher regenerative cell potency and greater ECM support compared to those obtained with common enzymatic digestion methods. Further studies are being carried out to optimize the cell counts and to determine long-term clinical outcomes.