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Strawberry DNA Extraction

Cells are the basic unit of life and make up all plants, animals and bacteria. Deoxyribonucleic acid, or DNA, is the molecule that controls everything that happens in the cell. DNA contains instructions that direct the activities of cells and, ultimately, the body. With only a few household items, you can extract DNA from strawberries just about anywhere! Extracting DNA from strawberries is super easy, allowing you to learn about genomics. Join NHGRI Director Eric Green and social media specialist Jenny Montooth as they show you step-by-step how to extract DNA from strawberries using materials available in your kitchen! Download printable instructions from: https://www.genome.gov/strawberry-DNA Materials needed: A resealable plastic bag 2 strawberries (fresh or frozen, but remove the green leaves) 2 tsp. dish detergent 1 tsp. salt ½ cup water 2 plastic cups 1 coffee filter ½ cup cold rubbing alcohol 1 coffee stirrer and 1 scientist (you!) Steps 1 & 2: Place strawberries in a plastic bag. After closing the bag, SMASH the strawberries for ~2 minutes until nice and smashed! Step 3: Make DNA extraction solution by adding the following to the first plastic cup: 2 tsp. dish detergent 1 tsp. salt 1/2 cup water Steps 3 & 4: After gently mixing the extraction solution (until salt is dissolved), pour it into the bag of smashed strawberries. Step 5: Reseal the bag and gently massage and mix the mixture for a minute. Try to avoid making too many soap bubbles! Steps 6 and 7: Place the coffee filter over the opening of the second plastic cup. open the plastic bag, and pour the liquid onto the coffee filter. Then wait for the liquid to drip through the coffee filter into the plastic cup, leaving the strawberry schmutz on the coffee filter. Step 8: You’re almost there! Pour an equal amount of cold rubbing alcohol into the liquid in the second plastic cup. Step 9: Gently move the cup around in circles and watch the whitish snot-like material form in the upper layer of liquid -- that is the strawberry DNA! Step 10: Use the coffee stirrer to pick up strawberry DNA out of the liquid. Credits: Producer/Writer/Director/Co-Star: Jenny Montooth, M.A., NHGRI Videographer/Animator/Editor: Ernesto Del Aguila III, NHGRI Cameraperson/Audio: Alvaro Encinas, NHGRI Set and Prop Design: Britny Kish, NHGRI Executive Producer: Sarah Bates, M.A., M.S., NHGRI Scientist: Eric Green, M.D., Ph.D., NHGRI