Measuring TCR-pMHC Binding In Situ Using FRET-Based Microscopy Assay l Protocol Preview скачать в хорошем качестве

Measuring TCR-pMHC Binding In Situ Using FRET-Based Microscopy Assay l Protocol Preview

Watch the Full Video at https://www.jove.com/v/53157/measurin.... Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay - a 2 minute Preview of the Experimental Protocol Markus Axmann, Gerhard J. Schütz, Johannes B. Huppa Vienna University of Technology, Institute for Applied Physics - Biophysics; Medical University of Vienna, Institute for Hygiene and Applied Immunology; This manuscript describes how to conduct (single molecule) Förster Resonance Energy Transfer (FRET)- based assays to measure the binding dynamics between T-cell antigen receptor (TCR) and antigenic peptide-loaded MHC molecules as they occur within the immunological synapse of a T-cell in contact with a functionalized planar supported lipid bilayer. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments