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Western Blot Protocol

Looking for a way to visualize a protein from cells or tissue samples? Maybe using... the dreaded Western Blot? Well fear not! Meghan from Addgene is here to make sure you'll be running, transferring, and chemiluminescing like a pro by the time you finish this video. If you're looking for more information and our full written protocol on Western Blots, visit https://www.addgene.org/protocols/wes... ___________________________________________________________________________ 00:00 - Intro 00:47 - Materials 02:00 - Preparing Samples 03:57 - Preparing the Gel 07:21 - Using the Transfer Apparatus 10:20 - Primary Antibody Incubation 12:09 - Secondary Antibody Incubation 13:00 - Prepare the Detection Reagent ___________________________________________________________________________ Credits: Featuring: Meghan Rego Written By: Meghan Rego, Rachel Leeson, & Quintin Marcelino Directed, Animated, & Edited by: Quintin Marcelino Videography by: Quintin Marcelino Sound Design by: Quintin Marcelino Designs by: Jason Snair Addition Designs by: Quintin Marcelino Music by: Anno Domini Beats & Bail Bonds, courtesy Youtube Audio Library. Disclaimer: The SOP presented here has been designed by the Addgene nonprofit plasmid repository and is being shared outside of Addgene for informational purposes only. If you choose to reuse or repurpose this SOP in another location, please note that you do so at your own risk; you should ensure that any local guidance is also adhered to. None of the authors, contributors, administrators, or anyone else associated with Addgene, can be held responsible for your use of the information contained in or linked to from these web pages.