Antibodies in the Lab - Addgene's Antibodies 101 скачать в хорошем качестве

Antibodies in the Lab - Addgene's Antibodies 101

Antibodies are powerful molecules, not just for your immune system, but for your research, too! But with so many considerations to make and so many ways scientists use antibodies in the lab, it can be hard to know where to get started. The good news is: we’re here to help! This video covers some of the ways scientists use antibodies in the lab to help you understand how you could use antibodies in your own experiments. For even more, check out Addgene’s Antibody Guide and the Addgene blog. https://www.addgene.org/guides/antibo... https://www.addgene.org/educational-r... https://blog.addgene.org/ https://datahub.addgene.org/antibody/ ______________________________________________________________ 00:00 - Introduction 00:43 - How Scientists Use Antibodies 01:24 - Types of Antibodies 01:35 - Polyclonal Antibodies 02:00 - Monoclonal Antibodies 02:28 - How to “See” Antibodies 03:42 - Quantify 04:08 - ELISAs 05:17 - Western Blots 06:44 - Capture & Immunoprecipitation 08:10 - Visualization - ICC and IHC 08:58 - Multiplexing 09:12 - Identifying - Flow Cytometry and FACs 10:14 - Differences in Flow Cytometry and FACs 10:56 - More Resources and Wrapping Up ______________________________________________________________ Citations: Western Blot Data at 06:05 - Nie et al. An appropriate loading control for western blot analysis in animal models of myocardial ischemic infarction, Biochemistry and Biophysics Reports, 12 (2017). https://doi.org/10.1016/j.bbrep.2017..... Zhao et al. Identification of valid reference genes for mRNA and microRNA normalisation in prostate cancer cell lines. Sci Rep 8, 1949 (2018). https://doi.org/10.1038/s41598-018-19... Flow Data at 10:15 - Boix, F., Llorente, S., Eguía, J., Gonzalez-Martinez, G., Alfaro, R., Galián Megías, J. A., Campillo, J., Moya-Quiles, M., Minguela Puras, A., Pons, J. A., & Muro, M. (2018). In vitro intracellular IFNγ, IL-17 and IL-10 producing T cells correlates with the occurrence of post-transplant opportunistic infection in liver and kidney recipients. World Journal of Transplantation, 8, 23–37. ______________________________________________________________ Credits: Written by: Nyla Naim, Ashley Waldron, Rachel Leeson & Quintin Marcelino Illustrations and Designs by: Jason Snair Directed, Animated, Edited, and Narrated by: Quintin Marcelino Additional Illustrations by: Quintin Marcelino Music and Sound Effects courtesy of Epidemic Sound Special Thanks to: Brook Pyhtila, Mike Lacy, Meghan Rego, Kate Harten, & Jo Dwyer