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Site-directed mutagenesis

Site-directed mutagenesis is a laboratory/PCR based technique used to introduce specific mutations into a DNA sequence. This method allows researchers to study the effects of mutations on the function of a gene or protein, and can be used to create new variants with desired properties. Site-directed mutagenesis can be performed using PCR-based or cloning-based methods, and has many applications in both basic research and biotechnology, including studying protein function and engineering enzymes with improved activity or specificity. There are various techniques involved are: Quick change method Overlap Primer based method inverse PCR based Megaprime based method Here in this lecture we will discuss on Quick change method and Overlap Primer based method. #SiteDirectedMutagenesis #SDM #Mutagenesis #ProteinEngineering #GeneticEngineering #MolecularBiology #PCR #Cloning #EnzymeEngineering #GenomeEditing #biotechnology #genetics #csir SDM lecture 2 :    • Site Directed Mutagenesis : Inverse and Me...