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Western Blot: Introduction to Immunodetection

The western blot is a widely used research technique that detects proteins using antibodies. One important step of a western blot is called, ‘immunodetection’ which is the unique step which uses primary and secondary antibodies to detect the protein of interest. The video lists the materials and reagents needed for immunodetection and covers how to conduct it, using footage from a laboratory at The Research Institute of St. Joe's Hamilton/McMaster University. This video was made by McMaster University students Aniqa Chaudhry, Brooke D'Mello, Ibrahim Ilahi, Kunwar Karim, Mubariz Maqsood, Adnan Moinuddin, and Julia Zhu in collaboration with the Demystifying Research McMaster Program. We would like to also acknowledge the contributions of Dr. Takuma Isshiki, who provided guidance in the process. Finally, we would like to also thank Dr. Kjetil Ask and the Ask Lab for the additional guidance, insights and support. Episode #1: Introduction to Western Blotting:    • Introduction to the Western Blot   Episode #2: SDS-PAGE:    • Western Blot: Introduction to SDS-PAGE   Episode #3: Wet Transfer:    • Western Blot: How to do a Wet Transfer   Episode #4: Electroblotting:    • Western Blot: Introduction to Electroblotting   Episode #5: Blocking:    • Western Blot: Introduction to Blocking   Episode #7: Results & Analysis:    • Western Blot: Result Interpretation   This video is for general and educational purposes only. Subscribe to the Demystifying Research McMaster channel. Copyright McMaster University 2022 #DemystifyingResearch #howtodoawesternblot #WesternBlot #immunodetection #immunoblotting References: JG;, Gallagher S;Winston SE;Fuller SA;Hurrell. “Immunoblotting and Immunodetection.” Current Protocols in Molecular Biology, U.S. National Library of Medicine, https://pubmed.ncbi.nlm.nih.gov/18633.... “Western Blotting Immunodetection Techniques.” Bio, https://www.bio-rad.com/fr-ca/applica.... RM;, Hnasko TS;Hnasko. “The Western Blot.” Methods in Molecular Biology (Clifton, N.J.), U.S. National Library of Medicine, https://pubmed.ncbi.nlm.nih.gov/26160....