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Restriction Digest Analysis

In this video, we will digest a plasmid of known identity with restriction enzymes, run the products on an agarose gel, and analyze the results. We’ll focus on identifying the bands produced under different conditions and use the results to make conclusions about the plasmid. Note: XbaI is effected by Dam methylation - see this blog post for more information http://blog.addgene.org/plasmids-101-... For the full written Restriction Digest Analysis protocol, visit: https://www.addgene.org/protocols/dia... ________________________________________________________________________________________________ 0:00 Intro 1:00 Determining Expected Results - Control 3:21 Determining Expected Results - Digestions 4:31 Run the Gel 5:40 Comparing the Data