aDNA extraction (Hundsdörfer, Patzold) скачать в хорошем качестве

aDNA extraction (Hundsdörfer, Patzold)

In this video we show you how to use the Monarch kit (NEB) for aDNA or historic DNA Isolation at Senckenberg Dresden. We take you ´backstage´ in our labratories and show you the process of DNA extraction step by step. Degradation of DNA into smaller fragments is caused by several factors including exposure to radiation (mainly UV), temperature, pH, chemicals used for specimen preservation, and free water, resulting in an inverse correlation between sample age and fragment length. These factors drive single-strand nicks and double-strand breaks, which leads to an accumulation of highly fragmented DNA and single-stranded DNA. Therefore, both highly fragmented DNA and single-stranded DNA (ssDNA) need to be targeted in DNA extraction from museum specimens. Customised non-commercial protocols (non-kit) were shown to be successful in DNA extraction from subfossil and museum specimens. However, they are very time consuming and thus not suitable for high throughput next generation sequencing (NGS) approaches. The transition of these protocols to a silica column-based approach would help to overcome this restriction and drive the study of century-old museum samples. New England Biolabs (2021) Monarch® PCR & DNA Cleanup Kit (5 μg). https://international.neb.com/product... Patzold, F., Zilli, A., & Hundsdoerfer, A. K. (2020) Advantages of an easy-to-use DNA extraction method for minimal-destructive analysis of collection specimens. PloS one, 15(7), e0235222. https://journals.plos.org/plosone/art... Patzold, F. (2020) ssDNA extraction protocol. dx.doi.org/10.17504/protocols.io.8m9hu96 Find out more about the the working group of Anna K. Hundsdoerfer on our website: https://www.senckenberg.de/en/institu... Senckenberg Dresden 2021