JONAH VENTURES: KNOWLEDGE IN SEQUENCE (CABW 2020 Presentation) скачать в хорошем качестве

JONAH VENTURES: KNOWLEDGE IN SEQUENCE (CABW 2020 Presentation)

JONAH VENTURES: KNOWLEDGE IN SEQUENCE Presenters: Joseph Craine - Jonah Ventures - https://jonahventures.com/ Peter Houpt- State Water Resources Control Board - www.waterboards.ca.gov/resources/oima/ Recorded at the 2020 California Aquatic Bioassessment Workgroup www.waterboards.ca.gov/water_issues/programs/swamp/bioassessment/cabw.html Jonah Ventures, founded in 2013 by two ecologists, helps other scientists answer ecological questions by sequencing environmental DNA (eDNA). Although initially focusing on reconstructing animal diets, Jonah Ventures has expanded to use quantitative PCR and next-generation sequencing with aquatic eDNA. For both citizen and professional scientists, we provide simple, pre-assembled kits to sample water. Users then collect meta-data on a phone app, send the sample to our lab where it is processed, and data are returned via an online data portal. Assays include source-tracking fecal bacteria, detecting the presence of individual aquatic species such as non-native species, and quantifying aquatic assemblages.Bioassessment is the next big challenge. Using Next Generation Sequencing, we can determine the presence or relative abundance of different species of phytoplankton, macroinvertebrates, mussels and fish in a water body. Once these data are compiled, it is a small step to score each species as indicators of environmental conditions such as eutrophication, and then calculate integrated environmental conditions. Before eDNA-based bioassessment is routine, work is needed to improve sequence reference libraries, score taxa relative to environmental conditions, and calculate eDNA-based bioassessment indices relative to reference conditions.