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Watch the Full Video at https://www.jove.com/v/53897/simultan.... Simultaneous Detection of c-Fos Activation from Mesolimbic and Mesocortical Dopamine Reward Sites Following Naive Sugar and Fat Ingestion in Rats - a 2 minute Preview of the Experimental Protocol Julie A. D. Dela Cruz, Tricia Coke, Richard J. Bodnar CUNY, New York, NY, Behavioral and Cognitive Neuroscience Cluster, Psychology Doctoral Program, The Graduate Center; CUNY, Flushing, NY, Department of Psychology, Queens College; CUNY, Flushing, NY, Behavioral and Cognitive Neuroscience Cluster, Psychology Doctoral Program, The Graduate Center; The goal of this study is to identify reward-related distributed brain networks by delineating a reliable immunohistological technique using cellular c-fos activation to measure simultaneous changes in dopamine pathways and terminal sites after novel ingestion of fat and sugar in rats. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments