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Spectrophotometry Spectrophotometry is a method that allows you to measure how much light a solution absorbs in a specific wavelength, with the aid of a spectrophotometer. This can be done using cuvettes or microplates, depending on the type of spectrophotometer that you have access to. The last component of each method is to prepare an appropriate standard curve where the absorbance values are presented on the y-axis and the protein concentration values are presented on the x-axis. Additional Resources BCA Workflow Course • BCA Workflow | Protein Concentration Best Practices Lesson • Best Practices and Material Requirements |... About LICORbio LICORbio offers imaging systems, analysis software, and IRDye® infrared dye reagents for quantitative protein and small animal imaging. LICORbio provides an integrated solution for quantitative Western blots with its Odyssey® Infrared Imaging Systems, Image Studio™ quantification software, Empiria Studio® analysis software for publication-ready data, reagents, unique IRDye® secondary antibodies, and online training courses. For chemiluminescent Western blots, the C-DiGit® Blot Scanner is a personal, portable imager. Use the Odyssey® XF or Odyssey M Imaging Systems for both chemiluminescence and near-infrared fluorescence Western blots. You can achieve superior small animal imaging results using BrightSite™ near-infrared fluorescent probes or bioluminescence on the Pearl® Trilogy imaging system. LICORbio Website https://www.licorbio.com/ Reagent Store https://shop.licorbio.com/reagents/ Image Studio™ Software https://www.licorbio.com/image-studio LinkedIn / licorbio Twitter / licorbio Facebook / licorbio