QC on High Content Imaging Datasets using CellProfiler and Fiji - AskErinDearBeth Episode 3 скачать в хорошем качестве

QC on High Content Imaging Datasets using CellProfiler and Fiji - AskErinDearBeth Episode 3

In this episode of Ask Erin/Dear Beth, Erin walks us through her favorite QC workflow for high content microscopy, using CellProfiler and Fiji (ImageJ) to make a representative montage of images that can be used for QC ~*~vibes~*~, or more specifically, to QC segmentation along with catching debris, confluency, or focus issues in the images. Don't ask Erin a question if you need a short answer, but the short answer to "what is high content microscopy" is “Anything that just doesn’t quite make sense for a human to sit down and click through the images themselves.” #science #openscience #microscopy #tutorial #opensource #biology #imageanalysis #segmentation #cellprofiler #Fiji #imagej ================================================== On Ask Erin/Dear Beth, bioimage analysis experts Beth Cimini and Erin Weisbart, of the Imaging Platform at the Broad Institute of MIT and Harvard, answer your image analysis questions! Whether it’s about tuning or troubleshooting, segmenting or measuring, Beth and Erin have opinions and by golly are they going to share them! Using mostly CellProfiler, but with expertise in many other image analysis softwares such as ImageJ/Fiji, Cellpose, Piximi, and more, no image analysis question is too hard, so ask away! Submit your own questions at broad.io/AskErinDearBeth Images and pipelines we’ve used are available for you to download and play with at https://ciminilab.github.io/AskErin_D...