PNAS 2020 Cozzarelli Prize Winner for Class IV: Biomedical Sciences скачать в хорошем качестве

PNAS 2020 Cozzarelli Prize Winner for Class IV: Biomedical Sciences

HIV-1 uncoats in the nucleus near sites of integration Ryan C. Burdick, Chenglei Li, MohamedHusen Munshi, Jonathan M. O. Rawson, Kunio Nagashima, Wei-Shau Hu, and Vinay K. Pathak During infection, the human immunodeficiency virus (HIV-1) must disassemble the capsid coating surrounding its viral DNA before incorporating the DNA into the host genome. However, the intracellular location and timing of viral uncoating have been unclear, with previous studies suggesting uncoating in the cytoplasm and outside the nucleus. The authors labeled the HIV-1 capsid protein and identified infectious virions, finding that viral cores enter the nucleus intact or nearly intact, and that uncoating occurs near the genomic integration site, within 1.5 hours of integration. The findings provide fundamental insights into HIV-1 behavior upon entering human cells and suggest that maintaining an intact core until shortly before integration may aid efficient viral replication and evasion of cellular immune responses. Read the award-winning research article: https://dx.doi.org/10.1073/pnas.19206...