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Apoptosis Assays (Notes included)

This podcast describes apoptosis assays, focusing on the Annexin V/PI staining method coupled with flow cytometry. Apoptosis, or programmed cell death, is explained as a crucial biological process with identifiable stages and markers. The presentation details how Annexin V and Propidium Iodide (PI) stain cells at different apoptotic stages, enabling researchers to quantify the percentage of apoptotic cells in a sample. Flow cytometry is highlighted as a key tool allowing high-throughput analysis of these markers. Finally, the podcast discusses the advantages and limitations of this technique and briefly mentions alternative apoptosis detection methods. Notes: https://github.com/PodcastLearn/Bioph...