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In this episode, Erin and Beth get a little goofy (what else is new!?) and ask a couple of different "Hard Hitting Questions" in a "man on the street"-style interview at the SBI2 conference 2025. Share your hot take in comments: What's your favorite CellProfiler feature? Widefield or confocal microscopy? #science #openscience #microscopy #tutorial #opensource #biology #imageanalysis #cellprofiler #manonthestreet ================================================== On Ask Erin/Dear Beth, bioimage analysis experts Beth Cimini and Erin Weisbart, of the Imaging Platform at the Broad Institute of MIT and Harvard, answer your image analysis questions! Whether it’s about tuning or troubleshooting, segmenting or measuring, Beth and Erin have opinions and by golly are they going to share them! Using mostly CellProfiler, but with expertise in many other image analysis softwares such as ImageJ/FIJI, Cellpose, Piximi, and more, no image analysis question is too hard, so ask away! Submit your own questions at broad.io/AskErinDearBeth Images and pipelines we’ve used are available for you to download and play with at https://ciminilab.github.io/AskErin_D...