Protein Microarray Part 2; Types of Protein Microarray, Applications and challenges скачать в хорошем качестве

Protein Microarray Part 2; Types of Protein Microarray, Applications and challenges

#biotechnology #protein #microarray #proteomics #ProteinMicroarray #FunctionalProteinMicroarrays #AnalyticalMicroarrays #ProteinExpression #MolecularBiology #ProteomicsResearch #ProteinDetection #BiomedicalResearch #Fluorescence #GeneExpression #CellLysateAnalysis #ProteinChip #ProteinInteractions #BiochemicalAnalysis #ProteinResearch #Biotechnology #MolecularBiology #Biomarkers #ProteinInteractions #DiagnosticTools #PostTranslationalModifications #DrugDiscovery #ProteinChipTechnology #FluorescenceDetection 00:00 - Recap of Part 1: What is Protein Microarray? 00:09 – Overview of Probes Used in Protein Microarrays 00:49 – Cell-Free Translation Systems & NAPPA 01:39 – Types of Protein Microarrays: Analytical vs. Functional 02:06 – Analytical Microarrays: Structure & Function 04:29 – Fluorescent Labeling and Detection in Analytical Microarrays 06:12 – Analyzing Protein Expression with Red/Green Fluorescence 09:01 – Introduction to Functional Protein Microarrays 10:22 - Introduction to functional protein microarrays vs. analytical microarrays 10:29 - What functional protein microarrays consist of 10:54 - Study of entire proteomes in a single experiment 11:09 - Two types of protein microarrays and their functions 11:23 - Extracting and labeling proteins for functional microarrays 11:46 - Fluorescence detection of protein interactions 12:07 - Examples of protein interactions studied with functional protein microarrays 13:28 - Applications of functional protein microarrays 14:01 - Identifying biomarkers and their role in disease diagnosis 14:52 - Post-translational modifications in protein analysis 15:37 - Host-pathogen interaction research with protein microarrays 16:36 - Challenges faced in protein microarray technology 17:43 - Issues with protein stability and secondary structure 18:02 - Difficulty in creating long shelf-life protein microarrays 19:04 - Challenges in quantifying protein levels and reducing background noise 19:25 - Identifying antibodies and isolating proteins for microarray 19:47 - Detecting both abundant and low-abundant proteins effectively The link to the 1st part is: Part 1:    • How Protein Microarrays Work: The Role of ...   Welcome back to part 2 of protein microarrays! In this video, we continue our exploration of protein microarrays, focusing on their types and applications in molecular biology. In part one, we discussed the basics of protein microarrays, the types of probes, and their sources, such as antibodies, aptamers, and recombinant DNA technology. Now, we explore two major types of protein microarrays: Analytical Microarrays and Functional Protein Microarrays. Analytical microarrays, or capture assays, are used to analyze protein presence and expression in various conditions, such as drug treatments. We explore how these microarrays can be used to compare protein expression and detect disease tissue profiling. On the other hand, functional protein microarrays are designed to study protein-protein, protein-DNA, protein-RNA interactions, and enzymatic activity. In this video, we'll break down the principles of both microarray types, their applications, and how they provide crucial insights into proteomics, gene expression, and molecular interactions. In this video, we dive deep into the world of protein microarrays, focusing on functional protein microarrays. Unlike analytical microarrays, functional protein microarrays are designed to study protein interactions and biochemical activities, offering insight into protein-protein, protein-DNA, and protein-RNA interactions. We explore the various applications of functional protein microarrays, such as detecting biomarkers, post-translational modifications, and host-pathogen interactions. Additionally, we discuss the challenges faced by protein microarrays, including issues with protein stability, surface attachment, and detection sensitivity. Learn how these cutting-edge technologies are used to understand proteomes and their significance in disease diagnosis and drug discovery. Keywords functional protein microarrays, protein-protein interactions, protein microarray technology, protein biochemical assays, protein chip applications, protein interaction studies, post-translational modifications, biomarker detection, host-pathogen interactions, proteomics research, protein labeling and fluorescence, protein microarray challenges, analytical vs functional protein microarrays, protein quantification in microarrays, Protein microarray, Analytical microarrays, Functional protein microarrays, Protein detection, Fluorescence imaging, Protein-DNA interaction, Protein expression profiling, Protein chip analysis, Biomedical applications of microarrays, Antibody immobilization, Aptamers in microarrays, Protein labeling techniques