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Watch the Full Video at https://www.jove.com/v/53392/measurin.... Measuring Physiological Responses of Drosophila Sensory Neurons to Lipid Pheromones Using Live Calcium Imaging - a 2 minute Preview of the Experimental Protocol Shruti Shankar, Meredith E.K. Calvert, Joanne Y. Yew Temasek Life Sciences Laboratory,; National University of Singapore, Department of Biological Science; Temasek Life Sciences Laboratory, Bioimaging and Biocomputing Facility; Gladstone Institutes, Histology and Light Microscopy Core; University of Hawai'i at Mānoa, Pacific Biosciences Research Center; The forelegs and proboscis of Drosophila contain a rich repertoire of gustatory sensory neurons. Here, we present a method using calcium imaging to measure physiological responses from sensory neurons in the foreleg and proboscis of live flies upon exogenous application of a gustatory pheromone. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments