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Learn about the monocular, binocular, ergo-monocular and double-header options for direct viewing and also about the modules for mounting a camera (C-mount, CS-mount and afocal eyepiece projection). PUMA is a DIY open source portable 3D printed microscope with augmented reality, fluorescence, phase contrast, polarization, epi-illumination and other advanced features. For more information see: https://onlinelibrary.wiley.com/doi/1... https://github.com/TadPath/PUMA CONTENTS ======== 00:08 What is an Ocular Head? 00:49 Fixed Angle Monocular 01:36 Using a C-mount Reduction Optic 02:22 The Ocular Cap Gap (OCG) 05:29 Ergo Monocular Head 06:35 The Mirror Block Tube Assembly 07:19 Binocular Head - Intro 09:20 [Support PUMA] 09:42 Bino Head as a Bincoular Viewer 15:20 Formula for IPD and OCG Settings 15:26 Formula for Extra Optics Compensation 18:45 Alignment Registration for Each Eye 22:08 Bino Head as a Double Header 23:26 Bino Head as an Ergo Monocular 25:30 Bino Head as a Camera Port 28:10 How To Build 28:43 C-Mount and CS-Mount Fittings 43:53 Concluding Remarks SUPPORT PUMA ============ PUMA is an open source microscopy project. You can help support the project by: 1. Become a Patron: / pumamicroscope 2. Donate via PayPal: https://www.paypal.com/donate/?hosted... 3. Simply subscribing to this YouTube channel, like, comment and share these videos. 4. Spread the word - post the news about PUMA and link to this YouTube channel on your social media sites and any other outreach method (tell your friends, colleagues, students or teachers and lecturers about PUMA, for example). 5. Consider purchasing your optics and related supplies from our affiliated online optics store, OptArc.co.uk ( https://www.optarc.co.uk/ ) --- PARTS AND TOOLS =============== For more details see the 'Bill_of_Materials' on the PUMA GitHub page ( https://github.com/TadPath/PUMA ) which also gives examples of where you can buy some of the more specialised items. The relevant worskheets in that document are: MD_Ocular MD_Monocular_C_Mount MD_Monocular_head MD_Binocular_head MD_Ergo_monocular_head For the epi-condenser light sink you print the 'DI_Epi_condenser' model from the Dominus Illuminator FreeCAD files For the interactive pointer you will need to build and fit the AR projector and PUMA Control Console - see the separate dedicated tutorials on those modules for details. Tools required are the usual 3D print finishing tools (craft knife, wire brush, fine grit sand paper, etc.) and Allen keys of appropriate sizes for the screws (1.5 mm for the M3 grub screws and M2 socket head screws, 2.5 mm for the M3 socket head screws). MODELS CREATED OR UPDATED FOR THIS VIDEO RELEASE ================================================ The following models were released on the GitHub repository just prior to this video being released - use these instead of any previous versions for the instructions in this video to 'work' and for optimal performance. Binocular: Cap_spacer_nut_arm - modified to have thicker plastic between the nut and the articulation and advised to use C'Condenser' Cura profile for toughter construction. Ocular_spacer_ring - new CM_Tube_short_c_adhesin - new CM_BB_Cover - new Outlet_upper - modified MB_Tube_Spacer_XpYmm - All spacers modified to have a slightly smaller outer diameter for easier fit in teh MBT and some new thicknesses made. Splitter_block - modified Outlet_lower_spacer - new Splitter_support - new Ocular_base_thread_L - modified, added receptacle for splitter support shelf Ocular_base_thread_R - modified, added receptacle for splitter support shelf Monocular: Ocular_Extn_CM_c_adhesin - updated EM_Block - new EM_Block_cover - new FOR FURTHER INFORMATION ======================= 1. Open access write-up about PUMA on the FreeCAD blog: https://blog.freecad.org/2023/02/13/a... 2. The official PUMA GitHub page where you can download the specs and source files to build or customise your own PUMA system: https://github.com/TadPath/PUMA 3. The scientific peer reviewed publication on PUMA that was published in 2021 in the Journal of Microscopy (a journal of the Royal Microscopical Society) available here: https://pubmed.ncbi.nlm.nih.gov/34151... https://onlinelibrary.wiley.com/doi/1... Thanks for your interest in the PUMA microscope system. PJT 23/03/22