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How to make a gel for protein SDS-PAGE

This video demonstrates how to pour a gel for protein SDS-PAGE. This is a commonly used method for visualizing the molecular weight and purity of proteins. Rather than purchasing precast gels for this purpose, you can prepare your own. The steps for gel pouring covered here are: 1.) Prepare the stock solutions and gel casting stands 2.) Test the gel setup for leaks 3.) Mix all reagents for Resolving and Stacking Gels except TEMED 4.) Add the TEMED and quickly pour Resolving Gel into mold 5.) Layer isopropanol on top of Resolving Gel and let polymerize 6.) Remove isopropanol and layer Stacking Gel into mold 7.) Immediately insert gel combs into Stacking Gel 8.) Once the gel has polymerized remove combs and store gel wet 9.) Use the gel to run SDS-PAGE experiment At Solidzymes we offer protein expression and purification services. Please pay us a visit. The full protocol and gel recipe is available on the Solidzymes website at this URL: https://solidzymes.com/wp-content/upl...