Confocal Imaging: Operating Inverted Zeiss LSM 880 Microscope using Zen Black 2.3 SP1 app. скачать в хорошем качестве

Confocal Imaging: Operating Inverted Zeiss LSM 880 Microscope using Zen Black 2.3 SP1 app.

Imaging parameters setting for confocal imaging 00:05 - Starting "Zen Black" app 00:19 - "Start System" wait for initialization to complete( takes about 40s) 00:50 - Power on lasers (01:07 to be precise) Locating your sample 01:46 - Locate tab 01:56 - Selecting/On/Of filters 02:27 - Placing slide on the stage 04:55 - Changing Reflected light level Imaging setup for Simultaneous scan for 3 Channel Imaging (Change track every line) 05:40 - Setting tracks 09:05 - Acquisition mode 09:16 - Select frame size, 09:23 - speed, 09:33 Averaging, 09:40 - Bit Depth, 09:45 - Scan direction 09:57 - Setting Pin hole size to 1 AU (10:09 to be precise) 10:24 - Setting Laser power and Detector sensitivity 14:16 - Taking an Image 14:46 - Saving an Image 16:21 - Using smart setup to set up light paths 22:25 - Using pre-set imaging parameters and setting laser power and detector sensitivity as per need 26:17 - Using settings from previously taken image (Reuse) 29:14 - Imaging with Z-Stacks 31:53 - Maximum intensity projection of Z-stacks 33:50 - Tile-Scan Imaging 36:15 - Turning off Lasers and closing Zen Black App