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The RCSB PDB Advanced Search tool allows users to build precise and complex queries of the PDB archive. Learn how to build complex queries, including 3D structural searches that are integrated with interactive Mol* visualization and how to use the Chemical Search option. After watching this video, you will be able to: • Determine the best search tool or tools to identify structures of interest • Select an attribute for searching (e.g., Organism, Experimental Method, Resolution) and add conditions to narrow results • Find structures that contain a similar nucleotide or protein sequence • Find structures with sequences that share small conserved regions (motifs) that have biological meaning • Find structures that share a similar 3D shape • Find structures that contain a similar 3D arrangement of residues with functional significance (catalytic or binding sites) • Search and explore experimental structures, integrative structures, and Computed Structure Models (CSMs) Video chapters: 00:00 Introduction: Protein Data Bank and RCSB PDB 01:55 Advanced Search For 3D Structure Discovery 03:41 Overview of Advanced Search Tools on RCSB.org 07:30 Accessing the Advanced Search 07:50 Introduction to Structure Attributes Search 09:27 Introduction to Sequence Similarity Search 12:35 Introduction to Sequence Motif Search 14:04 Introduction to 3D Similarity Search 16:50 Introduction to 3D Motif Search 24:00 Case Study: Trypsin Catalytic Site 26:33 Search Results Exploration 34:07 Chemical Search Capabilities Additional resources for this webinar are available on https://pdb101.rcsb.org/train/trainin... Explore all training courses at https://pdb101.rcsb.org/train/trainin...