Spatial Transcriptomics: Mapping Gene Activity in Tissue Location скачать в хорошем качестве

Spatial Transcriptomics: Mapping Gene Activity in Tissue Location

Spatial Transcriptomics (ST) revolutionizes molecular biology by mapping gene activity directly within intact tissue sections. Learn how ST overcomes the limitations of bulk and single-cell RNA sequencing, which destroy crucial spatial context. We detail sequencing-based methods like Visium and high-resolution imaging techniques such as Multiplexed Error-Robust Fluorescence In Situ Hybridization. Discover the technical principles of barcoded capture spots and multiplexed hybridization. Explore critical applications in oncology, mapping the tumor microenvironment, and neuroscience, resolving molecular atlases of the brain. Understand how ST data integration reveals spatially variable genes and cell-cell communication networks, driving the future of personalized medicine. 00:00: Beyond Bulk: Tissue Heterogeneity 01:02: Spatial Transcriptomics Defined 02:08: Visium: Barcoded Capture Spots 03:17: Bead-Based Spatial Mapping 04:13: Imaging: Multiplexed FISH Techniques 05:16: Data Integration and Visualization 06:11: Oncology: Tumor Microenvironment Mapping 07:00: Neuroscience and Developmental Biology 07:54: Multi-Omics and Future Directions