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VIDEO CORRECTION! The correct dilution for hydrogen peroxide is 0.03%, not 0.3% as said in the video. The measurement shown is correct, 1 ml 3% H2O2 into 100 mL agar. Sorry! Once you’ve mastered the basics of DIY mushroom cultivation and you’re ready to take a deep dive into the heart of mycology, making your own agar media and petri dishes is a must. In this in-depth video, you’ll discover the secret recipe to unlock infinite nutritious growth media for your fungus. Learn how to prepare, sterilize, pour, catalogue and store your very own petri dishes. Pick up lots of great tips and tricks along the way, including how to make hydrogen peroxide media (a great alternative to antibiotic agar), how to avoid senescence, get the most out of your mycelium and keep your fungus well-fed and happy for years to come. Once you’ve tackled the techniques in this video, you’ll be ready to charge ahead into the uncharted territory of advanced DIY mycology, including breeding, cloning and culture preservation. Next, check out our tutorial on sterile culture technique, Mycology Lab 101: • Mycology Lab 101: Agar Work, Cloning,... Made with love by Fungaia, a heart-centered, donation-based mushroom company: https://www.fungaia.life/ For the best, most reliable and super affordable mushroom genetics and liquid culture, visit our favorite culture library: True Blue Genetics https://www.TrueBlueGenetics.org/?cou... Discount code: BLAGAR ____________________________ Supplies List 1. Mushroom Genetics Gourmet and Medicinal Mushroom Cultures from Fungaia https://www.fungaia.life/shop/species/ The best liquid culture on the market: True Blue Genetics https://truebluegenetics.org/?coupon=... This is the last genetics provider you will ever need! 2. Ingredients Organic Gelidium Agar & Media Premix https://www.fungaia.life/shop/agar/ Malt Extract: https://amzn.to/3IX8LqA Nutritional Yeast: https://amzn.to/3PD7sRa Gypsum: https://amzn.to/3TBzyxt Activated Charcoal: https://amzn.to/3TVTxIP 3. Petri Dishes Sterile, Disposable Petri Dishes Pack of 25: https://amzn.to/3IU0pQD Case of 500: https://amzn.to/3PEVpmp Pyrex Petri Dishes https://amzn.to/4axywtq ____________________________ Recipes MEA 10 grams agar 10 grams malt extract pinch each gypsum & yeast 500 mL water PDA 10 grams agar 8 grams dextrose 3 grams potato flakes pinch each gypsum & yeast 500 mL water ____________________________ Other Useful Supplies The Fungaia Cube Kit https://www.fungaia.life/shop/kits/?q... An organic, all-in-one grow kit for dung-lover mushrooms Pre-Sterilized Grain Bags https://www.fungaia.life/shop/fertile... Pasteurized, Manure-Based Mushroom Substrate https://www.fungaia.life/shop/fertile... Monotubs and monotub filters: Microppose https://microppose.com/r?id=h776jn Discount code FUNGAIA Bulk Liquid Culture IV Bags https://www.fungaia.life/shop/spawn/?... ____________________________ More Videos from Fungaia Mycology Lab 101: Sterile Culture Technique • Mycology Lab 101: Agar Work, Cloning,... How to Grow Mushrooms the Easy Way: • How to Grow Mushrooms from Scratch th... How to Make Grain Spawn: • How to Make Grain Spawn: A Deep Dive ... How to make your own liquid culture • How to Make Liquid Culture the Easy W... Advanced liquid culture method • How to Make Liquid Culture: DIY Mushr... How to make your own injection port jar lids: • How to Make Injection Port Jar Lids f... ____________________________ Upcoming videos: Introduction to Mushroom Cultivation The Basics of Breeding Storing Genetics and Managing a Mushroom Culture Library Troubleshooting Contamination: Identification, Prevention and Problem-Solving Working with Mushroom Grow Bags Making Substrate for Dung-Loving Mushrooms Making Substrate for Wood-Loving Mushrooms Building a Home Lab The Laminar Flow Hood The Fungaia Flow Hood Kit DIY Bulk Pasteurizer DIY Cold Storage DIY Water Filtration Tyndallization: How to Make Grain Spawn without a Pressure Cooker Low-Tech Mushroom Cultivation Outdoor Mushroom Cultivation The Liquid Culture Revolution: 5 Ways Liquid Culture Will Change the World Fungaia's Bulk Liquid Culture System R.E.M.I.S.: The Remarkably Efficient Mass Inoculation System Myco-Materials Created by Paul Lynn. All rights reserved. (Made using some free stock footage.) © 2024 Alembic Enterprises LLC Walla Walla, Washington 0:00 Intro 3:18 Overview 3:35 Background 5:48 Side Note: No-Pour Agar Tek 6:26 Supplies 10:48 Ingredients 17:49 Recipes 23:05 Part 2: Prep & Sterilization 26:12 Part 3: Pouring Plates 29:14 Pro Tip #1: Color Coding 29:47 Pro Tip #2: Hydrogen Peroxide Plates 31:38 Drying 32:06 Pro Tip #3: Collating 32:34 Storage 33:44 Cleanup 34:08 Outro