Telomeric Repeat-containing RNA TERRA Analysis by Cancer Cell clones | Protocol Preview скачать в хорошем качестве

Telomeric Repeat-containing RNA TERRA Analysis by Cancer Cell clones | Protocol Preview

Watch the Full Video at https://www.jove.com/v/58790/generati.... Generation of Cancer Cell Clones to Visualize Telomeric Repeat-containing RNA TERRA Expressed from a Single Telomere in Living Cells - a 2 minute Preview of the Experimental Protocol Laura Avogaro, Claudio Oss Pegorar, Nicole Bettin, Emilio Cusanelli University of Trento, Laboratory of Cell Biology and Molecular Genetics, Department of Cellular, Computational and Integrative Biology - CIBIO; University of Trieste, Department of Life Sciences; Here, we present a protocol to generate cancer cell clones containing a MS2 sequence tag at a single subtelomere. This approach, relying on the MS2-GFP system, enables visualization of the endogenous transcripts of telomeric repeat-containing RNA (TERRA) expressed from a single telomere in living cells. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments