Скачать с ютуб Stefan Kubick - Cell-free systems for production & functional characterization of membrane proteins в хорошем качестве

Stefan Kubick - Cell-free systems for production & functional characterization of membrane proteins

Membrane proteins are key targets in both functional characterization and structural studies. Recently, cell-free systems, particularly eukaryotic ones, have emerged as efficient tools for rapidly producing a wide range of proteins. Many of these proteins require posttranslational modifications to function optimally. To date, several membrane proteins expressed in vivo closely mimic their authentic counterparts in function, antigenicity, and immunogenicity. Cultured eukaryotic cells perform essential modifications like glycosylation, phosphorylation, and palmitoylation. We have developed a method for creating translationally active eukaryotic lysates, preserving the membrane vesicles and their functionality, enabling advanced protein synthesis.