Part 1: Restriction enzyme digestion and DNA electrophoresis: data from Nobel Prize CRISPR research скачать в хорошем качестве

Part 1: Restriction enzyme digestion and DNA electrophoresis: data from Nobel Prize CRISPR research

0:00 Purpose of tutorial 0:38 Setting up the experiment 4:01 Digesting plasmid DNA 5:49 Recap of experimental procedure 6:49 Loading and running the gel 8:00 Reference to CRISPR exercise at Case It web site 8:39 Re-running gel on the Lab Bench (changing voltage, timer, agarose) This tutorial shows how the Case It simulation performs restriction enzyme digestion and gel electrophoresis. This example uses plasmid sequences from Nobel Prize-winning CRISPR research (https://www.caseitproject.org/analyzi.... The tutorial features the Data Screen, where analyses can be run quickly with more options than on the Lab Bench (that is featured in part 2 -    • Part 2:  Restriction enzyme digestion and ...  ). This free simulation software, available for both PC and Mac at https://www.caseitproject.org/, enables users to perform a variety of laboratory procedures on any DNA or protein sequence, including qPCR, SNP and expression microarrays, restriction enzyme digestion, gel electrophoresis, PCR, ELISA, Southern and Western blots, and dot blots (https://www.caseitproject.org/cases-a.... Case It won a Science Prize from the American Association of Science (AAAS) for its effectiveness in undergraduate biology and high school education. An essay describing the project was published in Science magazine (“Engaging Students in Molecular Biology via Case-Based Learning“ - https://www.science.org/doi/10.1126/s... ). Case It software was developed with funding from the National Science Foundation, which also supported extensive classroom testing and evaluation of project materials.