Optimized Multiplex qRT-PCR enables high-throughput microRNA profiling at the nanoliter scale скачать в хорошем качестве

Optimized Multiplex qRT-PCR enables high-throughput microRNA profiling at the nanoliter scale

Reference: https://app.jove.com/t/2552/high-thro... High-throughput profiling of microRNAs has become increasingly vital in the field of molecular biology, particularly for understanding gene regulation and expression. The implementation of optimized multiplex quantitative reverse transcription polymerase chain reaction (qRT-PCR) techniques at the nanoliter scale has significantly enhanced the efficiency and accuracy of microRNA analysis. Utilizing the Fluidigm Dynamic Array™ Integrated Fluidic Circuits (IFCs), researchers can conduct simultaneous amplification of multiple microRNA targets from minimal sample volumes, thereby maximizing the throughput while minimizing reagent consumption and costs. The Fluidigm Dynamic Array™ technology allows for the integration of numerous qRT-PCR reactions into a single platform, facilitating the analysis of a wide array of microRNAs in parallel. This multiplexing capability is particularly advantageous for studies requiring the examination of complex biological samples, as it enables the detection of subtle variations in microRNA expression profiles that may be indicative of specific physiological or pathological states. The precision of this method is further enhanced by the nanoliter-scale reaction volumes, which not only improve the sensitivity of the assay but also reduce the potential for contamination and sample loss. In summary, the optimized multiplex qRT-PCR approach on Fluidigm Dynamic Array™ IFCs represents a significant advancement in high-throughput microRNA profiling. By leveraging this innovative technology, researchers can achieve comprehensive and reliable microRNA expression analyses, paving the way for deeper insights into gene regulation mechanisms and their implications in various biological processes and diseases. This methodology not only streamlines the workflow but also opens new avenues for research in genomics and personalized medicine, where understanding microRNA dynamics is crucial for developing targeted therapeutic strategies.