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APPROACH TO PHERIPHERAL BLOOD SMEAR | HOW TO REPORT A GBP The slide is viewed at the body of the smear, usually beginning about one millimeter away from the tail (the monolayer part). The head of the smear should be avoided as the cell density is twice that seen at the tail. The head portion of the blood film might be of interest when investigating for presence of malaria parasites or microfilaria. The feathered end may be examined for platelet clumps and large cells like monocytes and blasts. Microscopy requires a skilled systematic approach. A quick assessment of a smear can be made within 3 minutes but an abnormal film would require longer time for wider view and differential cell counts. Peripheral blood smear can be used for estimation of manual blood counts. With the advent of automated cell counters which are more reliable and accurate, manual differential counts of white blood cells using PBF is gradually fading in routine haematology laboratory practice. However in resource deprived/ poor regions where automated counters are not readily available, assessing differential cell counts from PBF a valid option. In light of the above, the value of peripheral blood smear in assessing morphology and differential counting of blood cellular elements cannot be down-played. Morphology of the blood cells on a PBF smear is best discussed in line with each haemopoietic cell lineage. The distribution, size, shape, color, cellular inclusions of the red blood cell (RBC) and morphology of the other major cell lines should be carefully assessed. However, some abnormalities such as broken cells (smear or smudge cells) may be artefacts and should be taken into consideration when reporting. For estimating total leucocyte count, the smear cells seen must be included in the counts to avoid spurious results. Blood film should be interpreted alongside patient’s clinical details (history and physical examination). Results of other routine laboratory work-ups including full blood count, erythrocyte sedimentation rate, red cell indices should be part of the interpreting framework for reporting a PBF. #peripheralbloodsmear,#BloodPictureAnalysis,#HematologyLab,#MedicalDiagnosi,s #HematologicalAbnormalities,#BloodSmearInterpretation,#BloodCellsEvaluation,#ClinicalPathology,#HematologicDisorders,#BloodCellMorphology,#BloodCellCount,#BloodDiseases,#RedBloodCells,#WhiteBloodCells #Platelets,#BloodCellAbnormalities,#BloodMicroscopy,#Hematopatholog,.y #ClinicalLaboratoryScience,#MedicalResearch