Imaging FITC-dextran As Reporter or Regulated Exocytosis l Protocol Preview скачать в хорошем качестве

Imaging FITC-dextran As Reporter or Regulated Exocytosis l Protocol Preview

Watch the Full Video at https://www.jove.com/v/57936/imaging-.... Imaging FITC-dextran as a Reporter for Regulated Exocytosis - a 2 minute Preview of the Experimental Protocol Ofir Klein, Amit Roded, Koret Hirschberg, Mitsunori Fukuda, Stephen J. Galli, Ronit Sagi-Eisenberg Tel Aviv University, Department of Cell and Developmental Biology, Sackler Faculty of Medicine; Tel Aviv University, Department of Pathology, Sackler Faculty of Medicine; Tohoku University, Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences; Stanford University, Departments of Pathology and of Microbiology and Immunology and Sean N. Parker Center for Allergy and Asthma Research, School of Medicine; Here we detail a method for live cell imaging of regulated exocytosis. This method utilizes FITC-dextran, which accumulates in lysosome-related organelles, as a reporter. This simple method also allows distinguishing between different modes of regulated exocytosis in cells that are difficult to manipulate genetically. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments